Post-mortem Magnetic Resonance Imaging of Degenerating and Reorganizing White Matter in Post-stroke Rodent Brain

Vera H Wielenga; Rick M Dijkhuizen; Annette Van der Toorn

doi:10.1007/978-1-0716-2926-0_12

Post-mortem Magnetic Resonance Imaging of Degenerating and Reorganizing White Matter in Post-stroke Rodent Brain

Methods Mol Biol. 2023:2616:153-168. doi: 10.1007/978-1-0716-2926-0_12.

Authors

Vera H Wielenga¹, Rick M Dijkhuizen¹, Annette Van der Toorn²

Affiliations

¹ Biomedical MR Imaging and Spectroscopy Group, Center for Image Sciences, University Medical Center Utrecht & Utrecht University, Utrecht, The Netherlands.
² Biomedical MR Imaging and Spectroscopy Group, Center for Image Sciences, University Medical Center Utrecht & Utrecht University, Utrecht, The Netherlands. [email protected].

PMID: 36715933
DOI: 10.1007/978-1-0716-2926-0_12

Abstract

Magnetic resonance imaging (MRI) allows noninvasive and non-destructive imaging of brain tissue. More specifically, the status of white matter fibers can be measured with diffusion-weighted MRI, enabling assessment of structural degeneration or remodeling of white matter tracts in diseased brain. Here, we describe the preparation of post-stroke rodent brain samples for post-mortem high-resolution 3D diffusion-weighted MR imaging, accompanied with guidelines for acquiring and processing the images.

Keywords: Diffusion-weighted MRI; Microstructure; Neuroimaging; Rodent brain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / diagnostic imaging
Brain / pathology
Magnetic Resonance Imaging / methods
Rodentia
Stroke* / diagnostic imaging
Stroke* / pathology
White Matter* / diagnostic imaging
White Matter* / pathology