Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

David Diouf; Maria Rosaria Vitale; Johanna Eva Maria Zöller; Ana-Magdalena Pineau; Eva Klopocki; Catharina Hamann; Georg Christoph Ziegler; Tim Vanmierlo; Daniel Van den Hove; Klaus-Peter Lesch

doi:10.1016/j.scr.2023.103038

Generation of a ST3GAL3 null mutant induced pluripotent stem cell (iPSC) line (UKWMPi002-A-3) by CRISPR/Cas9 genome editing

Stem Cell Res. 2023 Mar:67:103038. doi: 10.1016/j.scr.2023.103038. Epub 2023 Jan 31.

Affiliations

¹ Division of Molecular Psychiatry, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany. Electronic address: [email protected].
² Division of Molecular Psychiatry, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany.
³ Institute of Human Genetics, Biocentre, University of Würzburg, Würzburg, Germany.
⁴ Division of Molecular Psychiatry, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany; Department of Psychiatry, Psychosomatics and Psychotherapy, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany.
⁵ Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, the Netherlands; Neuro-immune Connect and Repair Lab, Biomedical Research Institute, Hasselt University, Hasselt, Belgium.
⁶ Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, the Netherlands.
⁷ Division of Molecular Psychiatry, Center of Mental Health, University Hospital Würzburg, Würzburg, Germany; Department of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, Maastricht, the Netherlands. Electronic address: [email protected].

PMID: 36746102
DOI: 10.1016/j.scr.2023.103038

Abstract

Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems
Cell Differentiation
Cell Line
Gene Editing
Humans
Induced Pluripotent Stem Cells* / metabolism