Halomonas binhaiensis sp. nov., isolated from saline-alkali soil

A Gram-stain-negative, aerobic and rod-shaped bacterium, strain Y2R2^T, was isolated from a saline-alkali soil sample collected from Binhai New Area, Tianjin, PR China. Growth of strain Y2R2^T was observed at 10-45 °C (optimum, 30 °C), at pH 6.0-11.0 (optimum, pH 9.0) and in the presence of 0-15 % (w/v) NaCl (optimum, 9.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y2R2^T was affiliated with the genus Halomonas and showed the highest similarity to Halomonas huangheensis BJGMM-B45^T (99.0%) and Halomonas cupida DSM 4740^T (98.4%). The digital DNA-DNA hybridization and average nucleotide identity values of 21.0-22.8 % and 73.3-75.7 % with the closely related species H. huangheensis BJGMM-B45^T, H. cupida DSM 4740^T, H. ventosae AL12^T, H. stenophila N12^T and H. litopenaei SYSU ZJ2214^T were lower than the threshold recommended for species discrimination.The major respiratory quinone of strain Y2R2^T was Q-9 and the major cellular fatty acids consisted of C_{16 : 0}, C_{19 : 0} cyclo ω8c and summed feature 8 (C_{18 : 1} ω7c and/or C_{18 : 1} ω6c). The DNA G+C content of strain Y2R2^T was 57.0 mol%. On the basis of this polyphasic taxonomic study, strain Y2R2^T is considered to represent a novel species of the genus Halomonas, for which the name Halomonas binhaiensis sp. nov. is proposed. The type strain is Y2R2^T (=CGMCC 1.16974^T=KCTC 72578^T).