CRISPR/Cas9

Methods Mol Biol. 2023:2637:41-47. doi: 10.1007/978-1-0716-3016-7_3.

Abstract

CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and ease of use. Here, we introduce the construction of vectors and genome editing of the target gene in cells using the CRISPR/Cas9 system.

Keywords: CRISPR/Cas9; Cas9; gRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems* / genetics
  • Gene Editing*