Local displacement within the Achilles tendon induced by electrical stimulation of the single gastrocnemius muscles

Background: The Achilles tendon consists of three subtendons, but their functional meaning is still unknown. There are several approaches for the examination in-vivo using sonographic imaging, however, there is no approach for in-vivo examination with respect to the single subtendons of the m. triceps surae. The study's aim was to reveal the single subtendons of the m. triceps surae.

Methods: The Achilles tendon of 17 subjects was analysed. The muscles (m. gastrocnemius lateralis and medialis) were stimulated separately using neuromuscular electrical stimulation. The intensity of muscle contraction was controlled using electromyographic data. Sonographic videos of the Achilles tendon were recorded during muscle contraction. A speckle tracking algorithm was used to analyse the moving areas within the Achilles tendon during the initial phase of contraction.

Findings: The muscles were activated at 10-20% of the maximal M-wave. Isolated contraction of m. gastrocnemius lateralis led to local displacement in the lateral part of the Achilles tendon's cross-section whereas isolated contraction of m. gastrocnemius medialis led to displacement in the medial part and to a larger size of the area where initial displacement took place (m. gastrocnemius lateralis to medialis approximately 1:2).

Interpretation: The results demonstrate that isolated contractions of m. gastrocnemius lateralis and medialis lead to individual displacements which significantly differ. The differences in position and size of the area of the local displacement indicate an independent individual function. Unlike other studies generally investigating the AT in-vivo using muscle stimulation and ultrasonic imaging, this study investigated the AT's cross-section which had never been investigated before.