Longitudinal quantification of mouse gastric tumor organoid viability and growth using luminescence and microscopy

Riley J Morrow; Matthias Ernst; Ashleigh R Poh

doi:10.1016/j.xpro.2023.102110

Longitudinal quantification of mouse gastric tumor organoid viability and growth using luminescence and microscopy

STAR Protoc. 2023 Mar 17;4(1):102110. doi: 10.1016/j.xpro.2023.102110. Epub 2023 Feb 10.

Authors

Riley J Morrow¹, Matthias Ernst², Ashleigh R Poh³

Affiliations

¹ Olivia Newton-John Cancer Research Institute and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia.
² Olivia Newton-John Cancer Research Institute and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia. Electronic address: [email protected].
³ Olivia Newton-John Cancer Research Institute and School of Cancer Medicine, La Trobe University, Heidelberg, VIC 3084, Australia. Electronic address: [email protected].

Abstract

Tumor-derived organoids are valuable for testing anti-cancer drugs in vitro, but existing lysis-based protocols for viability measurement are laborious and restricted at a single time point. Here, we provide a lysis-free protocol for longitudinal and rapid assessment of mouse gastric tumor organoid viability and growth. We describe organoid plating, viability assessment via luminescence measurement, quantification of organoid growth by microscopy imaging, and treatment of organoids with test compounds to evaluate the effects on viability and growth at various time points.

Keywords: Cancer; Cell Biology; Cell culture; Cell-based Assays; Microscopy; Model Organisms; Organoids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents* / pharmacology
Luminescence
Mice
Microscopy
Organoids / pathology
Stomach Neoplasms*

Substances

Antineoplastic Agents