Immunofluorescence assay for demyelination, remyelination, and proliferation in an acute cuprizone mouse model

STAR Protoc. 2023 Mar 17;4(1):102072. doi: 10.1016/j.xpro.2023.102072. Epub 2023 Jan 30.

Abstract

Here, we present a protocol to assess demyelination in the corpus callosum of an acute cuprizone mouse model, which is routinely used to induce demyelination for studying myelin regeneration in the rodent brain. We describe the tracing of neural stem cells via intraperitoneal injection of tamoxifen into adult Gli1CreERT2;Ai9 mice and the induction of demyelination with cuprizone diet. We also detail EdU administration, cryosectioning of the mouse brain, EdU labeling, and immunofluorescence staining to examine proliferation and myelination. For complete details on the use and execution of this protocol, please refer to Radecki et al. (2020).1.

Keywords: Cell Biology; Microscopy; Model Organisms; Molecular/Chemical Probes; Neuroscience; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation
  • Cuprizone / toxicity
  • Demyelinating Diseases* / chemically induced
  • Fluorescent Antibody Technique
  • Mice
  • Remyelination*

Substances

  • Cuprizone