Breeding and Reproduction of the African Turquoise Killifish Nothobranchius furzeri

Jingxun Chen; Rishad C Khondker; Anne Brunet

doi:10.1101/pdb.prot107816

Breeding and Reproduction of the African Turquoise Killifish Nothobranchius furzeri

Cold Spring Harb Protoc. 2023 Jun 1;2023(6):pdb.prot107816. doi: 10.1101/pdb.prot107816.

Authors

Jingxun Chen¹, Rishad C Khondker¹, Anne Brunet^{2

3}

Affiliations

¹ Department of Genetics, Stanford University, Stanford, California 94305, USA.
² Department of Genetics, Stanford University, Stanford, California 94305, USA [email protected].
³ Glenn Laboratories for the Biology of Aging, Stanford University, Stanford, California 94305, USA.

PMID: 36863853
DOI: 10.1101/pdb.prot107816

Abstract

The successful breeding and reproduction of the African turquoise killifish Nothobranchius furzeri in a controlled laboratory setting are required to establish this fish species as a model system for studying vertebrate development and aging. Here, we describe a protocol to care for and hatch African turquoise killifish embryos, raise the juvenile fish to adulthood, and breed this species using sand as the breeding bedding. We also provide suggestions for generating a large quantity of good-quality embryos.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Aging
Animals
Cyprinodontiformes*
Fundulidae*
Killifishes
Reproduction

Supplementary concepts

Nothobranchius furzeri