Targeted Modifications of the Yeast Genome to Study Peroxisomes

Lakhan Ekal; Ewald Hettema

doi:10.1007/978-1-0716-3048-8_16

Targeted Modifications of the Yeast Genome to Study Peroxisomes

Methods Mol Biol. 2023:2643:217-232. doi: 10.1007/978-1-0716-3048-8_16.

Authors

Lakhan Ekal¹, Ewald Hettema²

Affiliations

¹ European Molecular Biology Laboratory, Hamburg, Germany. [email protected].
² Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield, UK.

PMID: 36952189
DOI: 10.1007/978-1-0716-3048-8_16

Abstract

PCR-based gene targeting enables rapid alteration of the Saccharomyces cerevisiae genome. Here we describe how this method can be applied for directed gene deletions, epitope and fluorescence protein tagging, and conditional gene expression, with a specific focus on peroxisomal proteins.

Keywords: Conditional gene/protein expression; Gene deletion; PCR-based tagging; S. cerevisiae.

MeSH terms

Gene Targeting
Peroxisomes / genetics
Peroxisomes / metabolism
Polymerase Chain Reaction / methods
Saccharomyces cerevisiae Proteins* / genetics
Saccharomyces cerevisiae Proteins* / metabolism
Saccharomyces cerevisiae* / genetics
Saccharomyces cerevisiae* / metabolism

Substances

Saccharomyces cerevisiae Proteins