Gene Targeting in Rat Embryonic Stem Cells

Hongsheng Men; Daniel J Davis; Elizabeth C Bryda

doi:10.1007/978-1-0716-2990-1_15

Gene Targeting in Rat Embryonic Stem Cells

Methods Mol Biol. 2023:2631:341-353. doi: 10.1007/978-1-0716-2990-1_15.

Authors

Hongsheng Men¹, Daniel J Davis², Elizabeth C Bryda^{3

2}

Affiliations

¹ Rat Resource and Research Center, University of Missouri, Columbia, MO, USA. [email protected].
² Animal Modeling Core, University of Missouri, Columbia, MO, USA.
³ Rat Resource and Research Center, University of Missouri, Columbia, MO, USA.

PMID: 36995676
DOI: 10.1007/978-1-0716-2990-1_15

Abstract

Rat germline-competent embryonic stem (ES) cell lines have been available since 2008, and rat models with targeted mutations have been successfully generated using ES cell-based genome targeting technology. This chapter will focus on the procedures of gene targeting in rat ES cells.

Keywords: ES cell selection; Gene targeting; Nucleofection; Rat ES cells.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Line
Embryonic Stem Cells*
Gene Targeting*
Genome
Germ Cells
Rats

Grants and funding

P40 OD011062/OD/NIH HHS/United States