Rat Embryonic Stem Cell Transgenesis

Elizabeth C Bryda; Hongsheng Men; Barbara J Stone

doi:10.1007/978-1-0716-2990-1_16

Rat Embryonic Stem Cell Transgenesis

Methods Mol Biol. 2023:2631:355-370. doi: 10.1007/978-1-0716-2990-1_16.

Authors

Elizabeth C Bryda¹, Hongsheng Men², Barbara J Stone³

Affiliations

¹ University of Missouri, Rat Resource and Research Center, Columbia, MO, USA. [email protected].
² University of Missouri, Rat Resource and Research Center, Columbia, MO, USA.
³ ParaTechs Corporation, Lexington, KY, USA.

PMID: 36995677
DOI: 10.1007/978-1-0716-2990-1_16

Abstract

The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.

Keywords: Blastocyst; Chimera; Embryo transfer; Embryonic stem cells; NSET; Rat; Transgenesis.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocyst*
Cell Line
Embryo Transfer / methods
Embryonic Stem Cells* / metabolism
Gene Transfer Techniques
Rats

Abstract

Publication types

MeSH terms

Grants and funding