Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease

Matthias Jung; Carla Hartmann; Toni Ehrhardt; Lisa-Maria Peter; Chaudhry Luqman Abid; Bernadette Harwardt; Jana Hirschfeld; Claudia Claus; Undine Haferkamp; Ole Pless; Marina Nastainczyk-Wulf; Astrid Kehlen; Dietmar Schlote; Insa S Schroder; Dan Rujescu

doi:10.1016/j.scr.2023.103072

Generation of a set of induced pluripotent stem cell lines from two Alzheimer disease patients carrying APOE4 (MLUi007-J; MLUi008-A) and healthy old donors carrying APOE3 (MLUi009-A; MLUi010-B) to study APOE in aging and disease

Stem Cell Res. 2023 Jun:69:103072. doi: 10.1016/j.scr.2023.103072. Epub 2023 Mar 15.

Authors

Affiliations

¹ Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany. Electronic address: [email protected].
² Institute for Physiological Chemistry, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06114 Halle (Saale), Germany.
³ Insitute for Medical Microbiology and Virology, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany.
⁴ Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Discovery Research ScreeningPort, 22525 Hamburg, Germany.
⁵ Institute for Forensic Medicine, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06112 Halle (Saale), Germany.
⁶ Department of Laboratory Medicine, Unit III, Molecular Diagnostic Section, Halle University Hospital, 06097 Halle (Saale), Germany.
⁷ Human Genetics Department, Medical Faculty of the Martin Luther University Halle-Wittenberg, 06112 Halle (Saale), Germany.
⁸ Department of Biophysics, GSI Helmholtz Center for Heavy Ion Research, 64291 Darmstadt, Germany.
⁹ Department of Psychiatry and Psychotherapy, Division of General Psychiatry, Medical University of Vienna, 1090 Vienna, Austria.

PMID: 37001364
DOI: 10.1016/j.scr.2023.103072

Abstract

Late-onset Alzheimer disease (LOAD) is the most frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for LOAD. Four human induced pluripotent stem cell (iPSC) lines MLUi007-J, MLUi008-B, MLUi009-A, and MLUi010-B were generated from LOAD patients and healthy matched donors by reprogramming of B-lymphoblastoid cells (B-LCLs) with episomal plasmids. The application of B-LCLs holds a great promise to model LOAD and other diseases because they can easily be generated from primary peripheral blood mononuclear cells (PBMCs) by infection with the Epstein-Barr virus (EBV).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging
Alzheimer Disease* / genetics
Alzheimer Disease* / metabolism
Apolipoprotein E3
Apolipoprotein E4 / genetics
Apolipoprotein E4 / metabolism
Epstein-Barr Virus Infections* / metabolism
Herpesvirus 4, Human
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear
Neurodegenerative Diseases* / metabolism

Substances

Apolipoprotein E4
Apolipoprotein E3