Volume EM: a quiet revolution takes shape

Lucy M Collinson; Carles Bosch; Anwen Bullen; Jemima J Burden; Raffaella Carzaniga; Cheng Cheng; Michele C Darrow; Georgina Fletcher; Errin Johnson; Kedar Narayan; Christopher J Peddie; Martyn Winn; Charles Wood; Ardan Patwardhan; Gerard J Kleywegt; Paul Verkade

doi:10.1038/s41592-023-01861-8

Volume EM: a quiet revolution takes shape

Nat Methods. 2023 Jun;20(6):777-782. doi: 10.1038/s41592-023-01861-8.

Authors

Lucy M Collinson¹, Carles Bosch², Anwen Bullen³, Jemima J Burden⁴, Raffaella Carzaniga⁵, Cheng Cheng⁶, Michele C Darrow^{7

8}, Georgina Fletcher⁹, Errin Johnson¹⁰, Kedar Narayan^{11

12}, Christopher J Peddie¹³, Martyn Winn¹⁴, Charles Wood¹⁵, Ardan Patwardhan¹⁶, Gerard J Kleywegt¹⁶, Paul Verkade¹⁷

Affiliations

¹ Electron Microscopy Science Technology Platform, Francis Crick Institute, London, UK. [email protected].
² Sensory Circuits and Neurotechnology Laboratory, Francis Crick Institute, London, UK.
³ UCL Ear Institute, University College London, London, UK.
⁴ Laboratory for Molecular Cell Biology, University College London, London, UK.
⁵ Zeiss Research Microscopy Solutions, Carl Zeiss Ltd, Zeiss Group, Cambourne, UK.
⁶ ConnectomX Ltd, Oxford, UK.
⁷ Artificial Intelligence & Informatics, The Rosalind Franklin Institute, Didcot, UK.
⁸ SPT Labtech Ltd., Melbourn, UK.
⁹ BioImagingUK, Oxford, UK.
¹⁰ Dunn School Bioimaging Facility, Sir William Dunn School of Pathology, Oxford University, Oxford, UK.
¹¹ Center for Molecular Microscopy, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
¹² Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
¹³ Electron Microscopy Science Technology Platform, Francis Crick Institute, London, UK.
¹⁴ UKRI-STFC, Rutherford Appleton Laboratory, Didcot, UK.
¹⁵ Future Technology Centre, School of Mechanical and Design Engineering, University of Portsmouth, Portsmouth, UK.
¹⁶ European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, UK.
¹⁷ School of Biochemistry, University of Bristol, Bristol, UK. [email protected].

Abstract

Volume Electron Microscopy is a group of techniques that reveal the 3D ultrastructure of cells and tissues through volumes greater than 1 cubic micron. A burgeoning grass roots community effort is fast building the profile, and revealing the impact, of vEM technology in the life sciences and clinical research.

MeSH terms

Volume Electron Microscopy*

Abstract

MeSH terms

Grants and funding