Insecticide Residues Associated with Apple Orchard Treatments in the Mason Bee, Osmia cornifrons, and their Nests

Declines in bee populations, in part due to pesticides, especially insecticides, are of global concern. Although most studies have investigated insecticide residues in honeybees and bumblebees, few have focused on non-Apis solitary bees, which are considered essential pollinators in the field. Most non-Apis bees are solitary and build their nests in the ground or in tree holes, therefore insecticide exposure pathways would differ from those of honeybees and bumblebees. We analyzed the residues of 20 insecticides in Osmia cornifrons bees and their pollen-provisions and nesting materials, along with adult honeybees, soil, and wildflowers collected in four apple orchards in two regions in Japan. Few insecticides were common among adult bees, pollen-provisions, and wildflowers. Insecticides applied in orchards were detected at high frequency: chlorantraniliprole, flubendiamide, and diazinon were detected in almost all samples. Insecticides without a known history of application were detected from various samples at frequencies ranging from 0% to 100%. Even in orchards without a known history of insecticide application, clothianidin was detected in many sample types and at high concentration. A purple deadnettle sample had the highest concentration at 17.5 mg/kg. These results highlight the complexity of pathways of insecticide exposure to O. cornifrons in the environment because insecticides may remain in the environment for long periods and wild bees may forage farther than previously known distances. Furthermore, each sample type contained different insecticides, so wild bees may have been exposed to different insecticides at different life stages. Future research should prioritize wide-scale measurements of insecticide residues in field conditions and toxicity testing with multiple exposures at different life stages of target species. Environ Toxicol Chem 2023;42:1564-1574. © 2023 SETAC.