Objective: To investigate the effect of scutellarin (SCU) on proliferation, cell cycle and apoptosis of acute myeloid leukemia (AML) cells and its related molecular mechanism.
Methods: Human AML HL-60 cells were cultured in vitro. The cells were treated with SCU at the concentration of 0, 2, 4, 8, 16, 32, 64 μmol/L, and the inhibition rate of cell proliferation was detected by CCK-8 method. Then HL-60 cells were treated with SCU at the concentration of 4, 8, 16 μmol/L, and the negative control group (NC group) was set. The cell cycle distribution and apoptosis were detected by flow cytometry, and the expression of cell cycle, apoptosis and JAK2/STAT3 pathway related proteins were detected by Western blot.
Results: SCU significantly inhibited the proliferation of HL-60 cells in a concentration- and time-dependent manner(r =0.958,r =0.971). Compared with NC group, the proportion of cells in G0/G1 phase and apoptosis rate of HL-60 cells in 4, 8, 16 μmol/L SCU group were significantly increased, and the proportion of cells in S phase was significantly decreased (P <0.05). The relative protein expression levels of p21, p53, caspase-3 and Bax were significantly increased, while the relative protein expression levels of CDK2, cyclin E and Bcl-2 were significantly decreased (P <0.05). The ratio of p-JAK2/JAK2 and p-STAT3/STAT3 were significantly decreased (P <0.05). The changes of above-mentioned indexes were concentration dependent.
Conclusion: SCU can inhibit the proliferation of AML cells, induce cell cycle arrest and apoptosis, and its mechanism may be related to the regulation of JAK2/STAT3 signaling pathway.
题目: 灯盏花乙素对急性髓系白血病细胞增殖的影响及作用机制研究.
目的: 探讨灯盏花乙素（SCU）对急性髓系白血病（AML）细胞增殖、周期与凋亡的影响及相关分子机制。.
方法: 体外培养人AML HL-60细胞,分别以0、2、4、8、16、32、64 μmol/L浓度的SCU处理HL-60细胞，采用CCK-8法检测细胞增殖抑制率。以4、8、16 μmol/L浓度的SCU处理HL-60细胞，并设置阴性对照组（NC组），采用流式细胞术检测细胞周期分布和细胞凋亡，Western blot法检测细胞周期、凋亡相关蛋白及JAK2/STAT3通路相关蛋白的表达。.
结果: SCU对HL-60细胞具有明显的增殖抑制作用，且增殖抑制作用呈浓度(r =0.958)、时间(r =0.971)依赖性。与NC组比较，SCU 4、8、16 μmol/L组HL-60细胞G0/G1期比例、细胞凋亡率均显著升高，S期比例均显著降低（P <0.05）；p21、p53、caspase-3、Bax蛋白相对表达量均显著升高，CDK2、cyclin E、Bcl-2蛋白相对表达量均显著降低（P <0.05），p-JAK2/JAK2、p-STAT3/STAT3比值均显著降低（P <0.05），且上述指标变化均呈浓度依赖性。.
结论: SCU可抑制AML细胞增殖，诱导细胞周期阻滞和细胞凋亡，其作用机制可能与调控JAK2/STAT3信号通路相关。.
Keywords: JAK2/STAT3 signaling pathway; acute myeloid leukemia; cell cycle; cell proliferation; scutellarin.