NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b

Biomol NMR Assign. 2023 Jun;17(1):135-142. doi: 10.1007/s12104-023-10132-8. Epub 2023 Apr 29.

Abstract

The splicing isoform b of human fibroblast growth factor 8 (FGF8b) is an important regulator of brain embryonic development. Here, we report the almost complete NMR chemical shift assignment of the backbone and aliphatic side chains of FGF8b. Obtained chemical shifts are in good agreement with the previously reported X-ray data, excluding the N-terminal gN helix, which apparently forms only in complex with the receptor. The reported data provide an NMR starting point for the investigation of FGF8b interaction with its receptors and with potential drugs or inhibitors.

Keywords: Dynamics; FGF; FGF8; FGF8b; FGFR; Fibroblast growth factor; Fibroblast growth factor receptor.; Motions; Structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fibroblast Growth Factor 8*
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Isoforms

Substances

  • Fibroblast Growth Factor 8
  • Protein Isoforms