Biogenic silver nanoparticle exhibits schistosomicidal activity in vitro and reduces the parasitic burden in experimental schistosomiasis mansoni

Mariana Barbosa Detoni; Bruna Taciane da Silva Bortoleti; Fernanda Tomiotto-Pellissier; Virgínia Marcia Concato; Manoela Daiele Gonçalves; Taylon Felipe Silva; Luryan Silvério Fidélis Ortiz; Amanda Caroliny Gomilde; Ana Carolina Jacob Rodrigues; Ricardo Luís Nascimento de Matos; Ana Paula Frederico Rodrigues Loureiro Bracarense; Andressa Maria Rorato Nascimento de Matos; Andréa Name Colado Simão; Thiago Hideo Endo; Renata Katsuko Takayama Kobayashi; Gerson Nakazato; Idessania Nazareth Costa; Ivete Conchon-Costa; Francisco José de Abreu Oliveira; Wander Rogério Pavanelli; Milena Menegazzo Miranda-Sapla

doi:10.1016/j.micinf.2023.105145

Biogenic silver nanoparticle exhibits schistosomicidal activity in vitro and reduces the parasitic burden in experimental schistosomiasis mansoni

Microbes Infect. 2023 Sep-Oct;25(7):105145. doi: 10.1016/j.micinf.2023.105145. Epub 2023 Apr 28.

Authors

Mariana Barbosa Detoni¹, Bruna Taciane da Silva Bortoleti², Fernanda Tomiotto-Pellissier², Virgínia Marcia Concato³, Manoela Daiele Gonçalves⁴, Taylon Felipe Silva³, Luryan Silvério Fidélis Ortiz³, Amanda Caroliny Gomilde³, Ana Carolina Jacob Rodrigues³, Ricardo Luís Nascimento de Matos⁵, Ana Paula Frederico Rodrigues Loureiro Bracarense⁵, Andressa Maria Rorato Nascimento de Matos⁵, Andréa Name Colado Simão⁶, Thiago Hideo Endo⁷, Renata Katsuko Takayama Kobayashi⁷, Gerson Nakazato⁷, Idessania Nazareth Costa³, Ivete Conchon-Costa³, Francisco José de Abreu Oliveira⁸, Wander Rogério Pavanelli³, Milena Menegazzo Miranda-Sapla⁹

Affiliations

¹ Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil. Electronic address: [email protected].
² Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil; Graduate Program in Biosciences and Biotechnology, Carlos Chagas Institute, Fiocruz, Curitiba, Paraná, Brazil.
³ Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil.
⁴ Laboratory of Biotransformation and Phytochemistry, Department of Chemistry, State University of Londrina, Londrina, Paraná, Brazil.
⁵ Laboratory of Animal Pathology, Department of Preventive Veterinary Medicine, State University of Londrina, Londrina, Paraná, Brazil.
⁶ Clinical Analysis and Toxicology, Department of Pathology, State University of Londrina, Londrina, Paraná, Brazil.
⁷ Laboratory of Basic and Applied Bacteriology, Department of Microbiology, State University of Londrina, Londrina, Paraná, Brazil.
⁸ Laboratory of Experimental Parasitology, Department of Pathological Sciences, State University of Londrina, Paraná, Brazil.
⁹ Laboratory of Immunoparasitology of Neglected Diseases and Cancer, Department of Pathological Sciences, State University of Londrina, Londrina, Paraná, Brazil; Postgraduate Program in Pharmaceutical Science, University of Vale Do Itajaí, Santa Catarina, Brazil. Electronic address: [email protected].

PMID: 37120010
DOI: 10.1016/j.micinf.2023.105145

Abstract

Schistosomiasis is a neglected tropical parasitic disease that affects millions of people, being the second most prevalent parasitic disease worldwide. The current treatment has limited effectiveness, drug-resistant strains, and is not effective in different stages of the disease. This study investigated the antischistosomal activity of biogenic silver nanoparticles (Bio-AgNp) against Schistosoma mansoni. Bio-AgNp presented direct schistosomicidal activity on newly transformed schistosomula causing plasma membrane permeabilization. In S. mansoni adult worms, reduced the viability and affected the motility, increasing oxidative stress parameters, and inducing plasma membrane permeabilization, loss of mitochondrial membrane potential, lipid bodies accumulation, and autophagic vacuoles formation. During the experimental schistosomiasis mansoni model, Bio AgNp restored body weight, reduced hepatosplenomegaly, and decrease the number of eggs and worms in feces and liver tissue. The treatment also ameliorates liver damage and reduces macrophage and neutrophil infiltrates. A reduction in count and size was evaluated in the granulomas, as well as a change to an exudative-proliferative phase, with a local increase of IFN-γ. Together our results showed that Bio-AgNp is a promising therapeutic candidate for studies of new therapeutic strategies against schistosomiasis.

Keywords: Anthelmintic; Nanotechnology; Schistosoma mansoni; Silver nanoparticles; Treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Humans
Metal Nanoparticles*
Schistosoma mansoni
Schistosomiasis mansoni* / drug therapy
Schistosomicides* / pharmacology
Schistosomicides* / therapeutic use
Silver / pharmacology

Substances

Schistosomicides
Silver