Mouse specific-locus test for the induction of heritable gene mutations by dibromochloropropane (DBCP)

Mutat Res. 1986 Jun;170(3):161-6. doi: 10.1016/0165-1218(86)90030-3.

Abstract

The nematocide DBCP (1,2-dibromo-3-chloropropane) produced negative results in a specific-locus test for gene-mutation induction in the germline of male (101 X C3H)F1 mice, most of which were treated with 5 daily intraperitoneal injections of 80 mg/kg (total exposure, 400 mg/kg); a few received lower exposures. For treated spermatogonial stem cells, the finding of 2 mutations among 39519 offspring--a rate almost identical to the control rate--rules out (at the 5% significance level) an induced mutation frequency greater than 2.0 times the historical control rate. From treated poststem-cell stages, no mutants were found among 6240 offspring, ruling out (at the 5% significance level) a multiple of 8.0 times the control for these cell types. A multiple rearrangement (7 chromosomes involved in 3 translocations) found in one of the mutants probably arose as a postmeiotic event not associated with the DBCP treatment. The fertility of DBCP-treated males was not disturbed, in keeping with the absence of germ-cell toxicity and dominant lethals found by other investigators in these mice, and in contrast to results in certain other species. While the treated (101 X C3H)F1 mice are Ah-responsive, other findings make it questionable whether biotransformation of DBCP to reactive intermediates is accomplished via the Ah-receptor system.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biotransformation
  • Female
  • Fertility / drug effects
  • Genes, Dominant / drug effects
  • Genes, Lethal / drug effects
  • Injections, Intraperitoneal
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mutagenicity Tests
  • Pregnancy
  • Propane / administration & dosage
  • Propane / analogs & derivatives*
  • Propane / metabolism
  • Propane / pharmacology
  • Translocation, Genetic

Substances

  • 1,2-dibromo-3-chloropropane
  • Propane