Establishment of bovine trophoblast stem cells

Yinjuan Wang; Hao Ming; Leqian Yu; Jie Li; Linkai Zhu; Hai-Xi Sun; Carlos A Pinzon-Arteaga; Jun Wu; Zongliang Jiang

doi:10.1016/j.celrep.2023.112439

Establishment of bovine trophoblast stem cells

Cell Rep. 2023 May 30;42(5):112439. doi: 10.1016/j.celrep.2023.112439. Epub 2023 May 4.

Authors

Yinjuan Wang¹, Hao Ming¹, Leqian Yu², Jie Li³, Linkai Zhu⁴, Hai-Xi Sun³, Carlos A Pinzon-Arteaga², Jun Wu⁵, Zongliang Jiang⁶

Affiliations

¹ School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70803, USA.
² Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
³ BGI-Shenzhen, Shenzhen, Guangdong 518083, China; BGI-Beijing, Beijing 102601, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China.
⁴ Department of Animal Sciences, University of Florida, Gainesville, FL 32608, USA.
⁵ Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: [email protected].
⁶ School of Animal Sciences, AgCenter, Louisiana State University, Baton Rouge, LA 70803, USA; Department of Animal Sciences, University of Florida, Gainesville, FL 32608, USA; Genetics Institute, University of Florida, Gainesville, FL 32610, USA. Electronic address: [email protected].

Abstract

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

Keywords: CP: Stem cell research; blastocyst; bovine; pluripotency; trophectoderm; trophoblast differentiation; trophoblast stem cells.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cell Differentiation / genetics
Female
Humans
Mice
Placentation
Pluripotent Stem Cells*
Pregnancy
Trophoblasts*

Abstract

Publication types

MeSH terms

Grants and funding