Isolation and characterization of a novel Enterococcus phage Phi_Eg_SY1

Qian Chen; Zhewen Dong; Tingting Ding; Qilin Yang; Chang Liu; Fang Yin; Huanlong Qin

doi:10.1016/j.virusres.2023.199132

Isolation and characterization of a novel Enterococcus phage Phi_Eg_SY1

Virus Res. 2023 Jul 15:332:199132. doi: 10.1016/j.virusres.2023.199132. Epub 2023 May 29.

Authors

Qian Chen¹, Zhewen Dong¹, Tingting Ding², Qilin Yang³, Chang Liu⁴, Fang Yin⁵, Huanlong Qin⁶

Affiliations

¹ Research Institute of Intestinal Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
² Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.
³ Research Institute of Intestinal Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China; Department of General Surgery, Shanghai Tenth Hospital, School of Clinical Medicine of Nanjing Medical University, Shanghai, China.
⁴ Department of Microbiology and Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
⁵ Research Institute of Intestinal Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: [email protected].
⁶ Research Institute of Intestinal Diseases, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China; Department of Gastrointestinal Surgery, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China. Electronic address: [email protected].

Abstract

Enterococcus gallinarum, a gut pathobiont, is an opportunistic pathogen that carries the risk of antibiotic resistance in the clinic and has been proven to drive autoimmunity in both mice and humans. Screening for novel bacteriophages targeting Enterococcus gallinarum is expected to provide a promising strategy for controlling such infections or regulating related chronic diseases. In the present study, we isolated a novel lytic Enterococcus gallinarum phage, Phi_Eg_SY1, which presents favourable thermostability and pH stability. Further assays indicated that Phi_Eg_SY1 can efficiently adsorb and lyse the host bacteria in vitro. Genomic and phylogenetic analyses suggested that Phi_Eg_SY1 does not contain virulence or lysogeny genes and presents a novel unassigned evolutionary lineage among the related dsDNA phages. Phi_Eg_SY1 is therefore considered to be suitable for further applications.

Keywords: Bacteriophage; Enterococcus gallinarum; Genome analyses.

MeSH terms

Animals
Bacteriophages* / genetics
Enterococcus / genetics
Humans
Lysogeny
Mice
Phylogeny

Supplementary concepts

Enterococcus gallinarum