Ratiometric near-infrared upconversion fluorescence sensor for selectively detecting and imaging of Al3

Caiping Ding; Yuting Gu; Weiwei Chen; Long Chen; Longhua Guo; Youju Huang

doi:10.1016/j.aca.2023.341297

Ratiometric near-infrared upconversion fluorescence sensor for selectively detecting and imaging of Al³

Anal Chim Acta. 2023 Jul 4:1263:341297. doi: 10.1016/j.aca.2023.341297. Epub 2023 May 2.

Authors

Caiping Ding¹, Yuting Gu¹, Weiwei Chen¹, Long Chen², Longhua Guo³, Youju Huang⁴

Affiliations

¹ Hangzhou Normal University, College of Material Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology, Department of Obstetrics and Gynecology, Affiliated Xiaoshan Hospital, Zhejiang Province, Hangzhou, 311121, PR China.
² Hangzhou Normal University, College of Material Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology, Department of Obstetrics and Gynecology, Affiliated Xiaoshan Hospital, Zhejiang Province, Hangzhou, 311121, PR China. Electronic address: [email protected].
³ College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, PR China.
⁴ Hangzhou Normal University, College of Material Chemistry and Chemical Engineering, Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Key Laboratory of Organosilicon Material Technology, Department of Obstetrics and Gynecology, Affiliated Xiaoshan Hospital, Zhejiang Province, Hangzhou, 311121, PR China. Electronic address: [email protected].

PMID: 37225340
DOI: 10.1016/j.aca.2023.341297

Abstract

Near-infrared (NIR) fluorescent probes provide extremely sensitive Al³⁺ detection for human health purposes. This research develops novel Al³⁺ response molecules (HCMPA) and NIR upconversion fluorescent nanocarriers (UCNPs), which respond to Al³⁺ through ratio NIR fluorescence. UCNPs improve photobleaching and visible light lack in specific HCMPA probes. Additionally, UCNPs are capable of ratio response, which will further enhance signal accuracy. The NIR ratiometric fluorescence sensing system has been successfully used to detect Al³⁺ within the range 0.1-1000 nM with an accuracy limit of 0.06 nM. Alternatively, a NIR ratiometric fluorescence sensing system integrated with a specific molecule can image Al³⁺ within cells. This study demonstrates that a NIR fluorescent probe is an effective and highly stable method of measuring Al³⁺ in cells.

Keywords: Al(3+); Imaging; Near-infrared fluorescent; Sensing; UCNPs.

MeSH terms

Fluorescence
Fluorescent Dyes*
Humans
Light*

Substances

Fluorescent Dyes