Objective: To develop asolvent extraction-direct mercury analyzer method for determination of methylmercury in urine. Methods: After the urinehydrolyzesd by hydrobromic acid, methylmercury was extracted by tolueneand reverse-extracted from L-cysteine solution, it was then detectedbydirect mercuryanalyzer. Results: The linear range was 0.2-50.0 μg/L, and the related coefficient was 0.9999. The relative standard deviations (RSD) within the group were 5.04%-6.64%, and the RSD between the group were 5.65%-8.11 %. The average recovery efficiencies were 85.4%-95.5%. The detection limitation was 0.0482 μg/L and the quantification concentrations was 0.1607 μg/L. Conclusion: The method, which has low detection limit, high sensitivity, easy to operate, is stability for the determination of methylmercury in urine.
目的： 建立尿中甲基汞的溶剂提取-直接测汞仪测定方法。 方法： 尿样水解后，甲基汞经甲苯萃取，再由L-半胱氨酸溶液反萃取出来，直接加入到直接测汞仪中测定。 结果： 尿中甲基汞浓度在0.2~ 50.0 μg/L线性关系良好，相关系数为0.999 9。方法批内精密度相对标准偏差（RSD）为5.04%~ 6.64%，批间精密度RSD为5.65%~8.11%，平均回收率分别为85.4%~95.5%，检出限为0.048 2 μg/L，定量下限为0.160 7 μg/L。 结论： 直接测汞仪测定尿中甲基汞的检出限低、灵敏度高、稳定性好、操作简便，可用于尿中甲基汞的测定。.
Keywords: Direct mercuryanalyzer; Methylmercury; Solvent extraction; Urine.