Genomic allele-specific base editing with imperfect gRNA

Xuxu Chen; Dongdong Zhao; Xueting Hou; Ju Li; Shiming Pu; Jidong Fei; Siwei Li; Zuping Zhou; Changhao Bi; Xueli Zhang

doi:10.1016/j.jgg.2023.05.010

Genomic allele-specific base editing with imperfect gRNA

J Genet Genomics. 2023 Oct;50(10):799-802. doi: 10.1016/j.jgg.2023.05.010. Epub 2023 Jun 1.

Authors

Xuxu Chen¹, Dongdong Zhao², Xueting Hou³, Ju Li⁴, Shiming Pu⁵, Jidong Fei², Siwei Li², Zuping Zhou⁶, Changhao Bi⁷, Xueli Zhang⁸

Affiliations

¹ School of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541006, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
² Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
³ School of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541006, China; Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China; Hubei College of Chinese Medicine, JingZhou, Hubei 434020, China.
⁴ College of Life Science, Tianjin Normal University, Tianjin 300387, China.
⁵ School of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541006, China; Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, Guangxi 541006, China.
⁶ School of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541006, China; Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, Guangxi 541006, China. Electronic address: [email protected].
⁷ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: [email protected].
⁸ Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China; Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China. Electronic address: [email protected].

PMID: 37269979
DOI: 10.1016/j.jgg.2023.05.010

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
CRISPR-Cas Systems / genetics
Gene Editing*
Genomics / methods
Humans
RNA, Guide, CRISPR-Cas Systems* / genetics

Substances

RNA, Guide, CRISPR-Cas Systems