Abstract
Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Colorimetry
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Dithiothreitol
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Indicators and Reagents
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Molybdenum
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Oxidation-Reduction
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Proteins / analysis*
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Spectrophotometry
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Tungsten
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Tungsten Compounds*
Substances
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Folin's phenol reagent
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Indicators and Reagents
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Proteins
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Tungsten Compounds
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Molybdenum
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Dithiothreitol
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Tungsten