Generation and characterization of a human induced pluripotent stem cell line (SDQLCHi052-A) from peripheral blood mononuclear cells derived from a healthy Chinese donor

Zirui Wan; Jingyun Guan; Yue Li; Haiyan Zhang; Bin Wang; Xiaomeng Yang; Xiaohua Jin; Xu Ma; Yi Liu

doi:10.1016/j.scr.2023.103139

Generation and characterization of a human induced pluripotent stem cell line (SDQLCHi052-A) from peripheral blood mononuclear cells derived from a healthy Chinese donor

Stem Cell Res. 2023 Sep:71:103139. doi: 10.1016/j.scr.2023.103139. Epub 2023 Jun 11.

Authors

Zirui Wan¹, Jingyun Guan², Yue Li², Haiyan Zhang², Bin Wang², Xiaomeng Yang², Xiaohua Jin³, Xu Ma⁴, Yi Liu⁵

Affiliations

¹ Pediatric Research Institute, Children's Hospital Affiliated to Shandong University, Ji'nan 250022, China; National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China; National Human Genetic Resources Center, Beijing 102206, China.
² Pediatric Research Institute, Children's Hospital Affiliated to Shandong University, Ji'nan 250022, China.
³ National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China; National Human Genetic Resources Center, Beijing 102206, China. Electronic address: [email protected].
⁴ National Research Institute for Family Planning, Beijing 100081, China; Graduate School, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100005, China; National Human Genetic Resources Center, Beijing 102206, China. Electronic address: [email protected].
⁵ Pediatric Research Institute, Children's Hospital Affiliated to Shandong University, Ji'nan 250022, China. Electronic address: [email protected].

PMID: 37320988
DOI: 10.1016/j.scr.2023.103139

Abstract

A non-integrated induced pluripotent stem cell (iPSC) line was generated derived from a healthy 20-day-old male using peripheral blood mononuclear cells. The established induced pluripotent stem cell line possesses a normal karyotype, expresses pluripotency markers and able to differentiate into all three germ layers in vitro. This cell line could serve as a health control or a basis for disease modeling, to explore molecular pathogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation
Cell Line*
East Asian People
Humans
Induced Pluripotent Stem Cells* / metabolism
Infant, Newborn
Leukocytes, Mononuclear / metabolism
Male