Immune-mediated inflammatory diseases (IMIDs) are commonly associated with complex coexisting conditions, and cardiovascular comorbidities are a common cause of mortality in systemic inflammation. Experimental models of disease provide an opportunity to dissect inflammatory mechanisms that promote damage to vascular tissues affected by comorbidity. Here, we describe methods to recover the thoracic aorta from mice during experimental inflammatory arthritis and assess vascular constriction responses by isometric tension myography. To complement the assessment of functional changes in the vasculature during inflammatory arthritis, we also outline a method to characterize vascular inflammation by immunohistochemistry.
Keywords: Cardiovascular comorbidity; Histopathology; Inflammatory arthritis; Isometric tension myography; Rheumatoid arthritis; Vascular contractile dysfunction.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.