Synthesis of DNA via deoxynucleoside H-phosphonate intermediates

B C Froehler; P G Ng; M D Matteucci

doi:10.1093/nar/14.13.5399

Synthesis of DNA via deoxynucleoside H-phosphonate intermediates

Nucleic Acids Res. 1986 Jul 11;14(13):5399-407. doi: 10.1093/nar/14.13.5399.

Authors

B C Froehler, P G Ng, M D Matteucci

Abstract

Deoxynucleoside H-phosphonates are used in the chemical synthesis of deoxyoligonucleotides up to 107 bases in length. The biological activity of the synthetic DNA is assessed by cloning into M13 and sequencing. An improved synthesis of protected deoxynucleoside H-phosphonates is also described.

MeSH terms

Chemical Phenomena
Chemistry
DNA / chemical synthesis*
Deoxyribonucleosides
Molecular Weight
Organophosphonates

Substances

Deoxyribonucleosides
Organophosphonates
DNA