Amplicon-Based Nanopore Sequencing of Patients Infected by the SARS-CoV-2 Omicron (B.1.1.529) Variant in India

Somesh Kumar; Avinash Lomash; Mohammed Faruq; Oves Siddiqui; Suresh Kumar; Seema Kapoor; Prashanth Suravajhala; Sunil K Polipalli

doi:10.1007/978-3-031-28012-2_15

Amplicon-Based Nanopore Sequencing of Patients Infected by the SARS-CoV-2 Omicron (B.1.1.529) Variant in India

Adv Exp Med Biol. 2023:1412:271-283. doi: 10.1007/978-3-031-28012-2_15.

Authors

Somesh Kumar^#¹, Avinash Lomash^#¹, Mohammed Faruq², Oves Siddiqui³, Suresh Kumar¹, Seema Kapoor¹, Prashanth Suravajhala^{4

5}, Sunil K Polipalli^#^{1

6}

Affiliations

¹ Genome Sequencing Laboratory, Lok Nayak Hospital, New Delhi, Delhi, India.
² Genomic & Molecular Medicine Division, CSIR-Institute of Genomics & Integrative Biology, New Delhi, India.
³ Department of Microbiology, Maulana Azad Medical College, New Delhi, India.
⁴ Bioclues.org, Hyderabad, India. [email protected].
⁵ Amrita School of Biotechnology, Amrita Vishwavidyapeetham, Clappana, Kerala, India. [email protected].
⁶ Bioclues.org, Hyderabad, India.

^# Contributed equally.

PMID: 37378773
DOI: 10.1007/978-3-031-28012-2_15

Abstract

We report the sequencing of SARS-CoV-2 Omicron variants from 75 patients, using nanopore long-read sequencing chemistry. These data show a range of mutations in spike glycoprotein that are both unique and common to other populations.

Keywords: COVID-19; Mutation; Omicron; SARS-CoV-2; Sequencing.

MeSH terms

COVID-19* / diagnosis
Humans
India / epidemiology
Mutation
Nanopore Sequencing*
SARS-CoV-2 / genetics

Supplementary concepts

SARS-CoV-2 variants