Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Hadar Bootz-Maoz; Ariel Simon; Sara Del Mare-Roumani; Yifat Bennet; Einat Toister; Hadar Romano; Danping Zheng; Sivan Amidror; Eran Elinav; Nissan Yissachar

doi:10.1038/s41522-023-00409-0

Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

NPJ Biofilms Microbiomes. 2023 Jul 3;9(1):44. doi: 10.1038/s41522-023-00409-0.

Authors

Hadar Bootz-Maoz^{1

2}, Ariel Simon^{1

2}, Sara Del Mare-Roumani^{1

2}, Yifat Bennet^{1

2}, Einat Toister^{1

2}, Hadar Romano^{1

2}, Danping Zheng³, Sivan Amidror^{1

2}, Eran Elinav^{3

4}, Nissan Yissachar^{5

6}

Affiliations

¹ The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
² Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel.
³ Systems Immunology Department, Weizmann Institute of Science, 234 Herzl Street, Rehovot, 7610001, Israel.
⁴ Microbiome & Cancer Division, Deutsches Krebsforschungszentrum (DKFZ), Neuenheimer Feld 280, 69120, Heidelberg, Germany.
⁵ The Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, 5290002, Israel. [email protected].
⁶ Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat-Gan, 5290002, Israel. [email protected].

Abstract

The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gastrointestinal Microbiome*
Host Microbial Interactions
Intestinal Mucosa*
Permeability