Macrophage migration inhibitory factor-mediated mast cell extracellular traps induce inflammatory responses upon Fusobacterium nucleatum infection

Hiroyuki Tada; Takashi Nishioka; Rina Ishiyama; Li-Ting Song; Sakura Onoue; Kazuyoshi Kawahara; Eiji Nemoto; Kenji Matsushita; Shunji Sugawara

doi:10.1016/j.bbrc.2023.06.060

Macrophage migration inhibitory factor-mediated mast cell extracellular traps induce inflammatory responses upon Fusobacterium nucleatum infection

Biochem Biophys Res Commun. 2023 Sep 24:674:90-96. doi: 10.1016/j.bbrc.2023.06.060. Epub 2023 Jun 29.

Authors

Hiroyuki Tada¹, Takashi Nishioka², Rina Ishiyama³, Li-Ting Song⁴, Sakura Onoue⁵, Kazuyoshi Kawahara⁵, Eiji Nemoto⁶, Kenji Matsushita⁷, Shunji Sugawara³

Affiliations

¹ Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan. Electronic address: [email protected].
² Liaison Center for Innovative Dentistry, Division of Advanced Education Development, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan; Oral and Maxillofacial Radiology, Tohoku University Hospital, Sendai 980-8575, Japan.
³ Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.
⁴ Division of Oral Immunology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan; Hospital of Stomatology, School of Dentistry, Tianjin Medical University, Tianjin 300070, China.
⁵ Department of Biosciences, College of Science and Engineering, Kanto Gakuin University, Yokohama 236-8501, Japan.
⁶ Division of Periodontology and Endodontology, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.
⁷ Department of Oral Disease Research, National Center for Geriatrics and Gerontology, Obu 474-8511, Japan.

PMID: 37413710
DOI: 10.1016/j.bbrc.2023.06.060

Abstract

Mast cell extracellular traps (MCETs) released by mast cells contribute to host defense. In this study, we investigated the effects of MCETs released from mast cells after infection with a periodontal pathogen Fusobacterium nucleatum. We found that F. nucleatum induced MCET release from mast cells, and that MCETs expressed macrophage migration inhibitory factor (MIF). Notably, MIF bound to MCETs induced proinflammatory cytokine production by monocytic cells. These findings suggest that MIF expressed on MCETs, released from mast cells upon infection with F. nucleatum, promotes inflammatory responses that may be associated with the pathogenesis of periodontal disease.

Keywords: Extracellular trap; Fusobacterium nucleatum; Lipopolysaccharide; Macrophage migration inhibitory factor; Mast cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Extracellular Traps* / metabolism
Fusobacterium nucleatum
Macrophage Migration-Inhibitory Factors* / metabolism
Mast Cells

Substances

Macrophage Migration-Inhibitory Factors