Subcellular positioning during cell division and cell plate formation in maize

Lindy A Allsman; Marschal A Bellinger; Vivian Huang; Matthew Duong; Alondra Contreras; Andrea N Romero; Benjamin Verboonen; Sukhmani Sidhu; Xiaoguo Zhang; Holly Steinkraus; Aimee N Uyehara; Stephanie E Martinez; Rosalie M Sinclair; Gabriela Salazar Soriano; Beatrice Diep; Dawson Byrd V; Alexander Noriega; Georgia Drakakaki; Anne W Sylvester; Carolyn G Rasmussen

doi:10.3389/fpls.2023.1204889

Subcellular positioning during cell division and cell plate formation in maize

Front Plant Sci. 2023 Jul 7:14:1204889. doi: 10.3389/fpls.2023.1204889. eCollection 2023.

Authors

Lindy A Allsman¹, Marschal A Bellinger¹, Vivian Huang¹, Matthew Duong¹, Alondra Contreras¹, Andrea N Romero¹, Benjamin Verboonen¹, Sukhmani Sidhu¹, Xiaoguo Zhang², Holly Steinkraus², Aimee N Uyehara¹, Stephanie E Martinez¹, Rosalie M Sinclair³, Gabriela Salazar Soriano¹, Beatrice Diep¹, Dawson Byrd V¹, Alexander Noriega¹, Georgia Drakakaki³, Anne W Sylvester², Carolyn G Rasmussen¹

Affiliations

¹ Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
² Department of Molecular Biology, University of Wyoming, Laramie, WY, United States.
³ Department of Plant Sciences, University of California, Davis, Davis, CA, United States.

Abstract

Introduction: During proliferative plant cell division, the new cell wall, called the cell plate, is first built in the middle of the cell and then expands outward to complete cytokinesis. This dynamic process requires coordinated movement and arrangement of the cytoskeleton and organelles.

Methods: Here we use live-cell markers to track the dynamic reorganization of microtubules, nuclei, endoplasmic reticulum, and endomembrane compartments during division and the formation of the cell plate in maize leaf epidermal cells.

Results: The microtubule plus-end localized protein END BINDING1 (EB1) highlighted increasing microtubule dynamicity during mitosis to support rapid changes in microtubule structures. The localization of the cell-plate specific syntaxin KNOLLE, several RAB-GTPases, as well as two plasma membrane localized proteins was assessed after treatment with the cytokinesis-specific callose-deposition inhibitor Endosidin7 (ES7) and the microtubule-disrupting herbicide chlorpropham (CIPC). While ES7 caused cell plate defects in Arabidopsis thaliana, it did not alter callose accumulation, or disrupt cell plate formation in maize. In contrast, CIPC treatment of maize epidermal cells occasionally produced irregular cell plates that split or fragmented, but did not otherwise disrupt the accumulation of cell-plate localized proteins.

Discussion: Together, these markers provide a robust suite of tools to examine subcellular trafficking and organellar organization during mitosis and cell plate formation in maize.

Keywords: cell plate; maize; microtubule; mitosis; phragmoplast.

Copyright © 2023 Allsman, Bellinger, Huang, Duong, Contreras, Romero, Verboonen, Sidhu, Zhang, Steinkraus, Uyehara, Martinez, Sinclair, Soriano, Diep, Byrd V., Noriega, Drakakaki, Sylvester and Rasmussen.

Grants and funding

Funding from NSF-MCB #1716972 and NSF-CAREER #1942734 to CGR. Funding from DOE-GAANN #P200A150300 to MB, Office of Undergraduate Education Minigrant Program, Bernarr J. Hall Agricultural Scholarship and UCR School of Medicine Medical Scholars Research Program to SS. AC, GS, DB, and AN were partially supported by USDA-NIFA U-SPARC 2017-38422-27135, BD was awarded a Chancellor’s Fellowship, MD was awarded a RISE fellowship from UCR, VH was awarded a Chancellor’s Minigrant. SM and AU received funding from NSF DBI-1922642. AS acknowledges funding from NSF-DBI 0501862 and NSF-IOS 1027445 as part of the Maize Cell Genomics project and gratefully acknowledges the contributions of Anding Luo. GD acknowledges funding from NSF-MCB #1818219.