Dual-channel MIRECL portable devices with impedance effect coupled smartphone and machine learning system for tyramine identification and quantification

Zhiwei Lu; Jun Qin; Chun Wu; Jiajian Yin; Mengmeng Sun; Gehong Su; Xianxing Wang; Yanying Wang; Jianshan Ye; Tao Liu; Hanbing Rao; Lin Feng

doi:10.1016/j.foodchem.2023.136920

Dual-channel MIRECL portable devices with impedance effect coupled smartphone and machine learning system for tyramine identification and quantification

Food Chem. 2023 Dec 15:429:136920. doi: 10.1016/j.foodchem.2023.136920. Epub 2023 Jul 20.

Authors

Zhiwei Lu¹, Jun Qin¹, Chun Wu¹, Jiajian Yin¹, Mengmeng Sun¹, Gehong Su¹, Xianxing Wang¹, Yanying Wang¹, Jianshan Ye², Tao Liu³, Hanbing Rao⁴, Lin Feng⁵

Affiliations

¹ College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an 625014, PR China.
² School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, PR China.
³ College of Information Engineering, Sichuan Agricultural University, Xinkang Road, Yucheng District, Ya'an 625014, PR China. Electronic address: [email protected].
⁴ College of Science, Sichuan Agricultural University, Xin Kang Road, Yucheng District, Ya'an 625014, PR China. Electronic address: [email protected].
⁵ Animal Nutrition Institute, Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China. Electronic address: [email protected].

PMID: 37487397
DOI: 10.1016/j.foodchem.2023.136920

Abstract

We designed a novel, portable, and visual dual-potential molecularly imprinted ratiometric electrochemiluminescence (MIRECL) sensor for tyramine (TYM) detection based on smartphone and deep learning-assisted optical devices. Molecularly imprinted polymer-Ce₂Sn₂O₇ (MIP-Ce₂Sn₂O₇) layers were fabricated by in-situ electropolymerization method as the capture and signal amplification probe. Oxygen vacancies in Ce₂Sn₂O₇ not only enhance the electrochemical redox capability but also accelerate the energy transfer, thereby enhancing the luminescence of cathode ECL. Under optimal conditions, the ECL signals of MIP-Ce₂Sn₂O₇ at the cathode and the anode response of Ru(bpy)₃²⁺ was reduced, thus a wide linear range from 0.01 μM to 1000 μM with the detection limit as low as 0.005 μM. Interestingly, combined with an artificial intelligence image recognition algorithm and the principle of optical signal reading by smartphone, the developed MIRECL sensor has been applied to the portable and visual determination of TYM in aquatic samples, and its practicability has been satisfactorily verified.

Keywords: Deep learning; Electrochemiluminescence; Molecularly imprinted polymer; Smartphone; Tyramine.

MeSH terms

Artificial Intelligence
Biosensing Techniques* / methods
Electric Impedance
Electrochemical Techniques / methods
Limit of Detection
Luminescent Measurements / methods
Machine Learning
Molecular Imprinting* / methods
Smartphone