Defluviimonas sediminis sp. nov., isolated from mangrove sediment

A Gram-stain-negative, facultatively aerobic, motile and ovoid- to rod-shaped bacterium, designated as FT324^T, was isolated from a surface mangrove sediment sample from Shenzhen, PR China. The taxonomic position of strain FT324^T was determined by a combination of phylogenetic, physiological, biochemical and chemotaxonomic analyses. Strain FT324^T grew at 20-40 °C (optimum, 30-37 °C), pH 5.0-9.0 (optimum, pH 8.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 1 %). Its full-length 16S rRNA gene sequence was the most similar to Frigidibacter oleivorans XJ4^T (97.4 %), followed by Defluviimonas denitrificans DSM 18921^T (96.62 %), Pseudothioclava arenosa CAU 1312^T (96.54 %) and Defluviimonas nitratireducens DL5-4^T (96.47 %). The major fatty acids (>10 %) of FT324^T were C_{19 : 0} cyclo ω8c (51.9 %) and summed feature 8(29.6 %). The predominant respiratory quinone was Q-10. Its polar lipid profile contained phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid, one unidentified lipid and two unidentified phospholipids. Its estimated genome size was 4 294 115 bp and the genomic DNA G+C content was 69.6 mol%. Based on its distinct phenotypic, chemotaxonomic and phylogenetic characteristics, strain FT324^T should represent a novel species of the genus Defluviimonas, for which the name Defluviimonas sediminis sp. nov. is proposed (=MCCC 1K07685^T=KCTC 92477^T).