Viral Vector-Induced Ocular Hypertension in Mice

J Cameron Millar; Yogapriya Sundaresan; Gulab S Zode; Abbot F Clark

doi:10.1007/978-1-0716-3409-7_9

Viral Vector-Induced Ocular Hypertension in Mice

Methods Mol Biol. 2023:2708:77-97. doi: 10.1007/978-1-0716-3409-7_9.

Authors

J Cameron Millar¹, Yogapriya Sundaresan^{1

2}, Gulab S Zode^{1

2}, Abbot F Clark³

Affiliations

¹ Department of Pharmacology & Neuroscience, North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA.
² Department of Ophthalmology, Gaven Herbert Eye Institute, UC Irvine, Irvine, CA, USA.
³ Department of Pharmacology & Neuroscience, North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, USA. [email protected].

PMID: 37558962
DOI: 10.1007/978-1-0716-3409-7_9

Abstract

Viral transduction of the mouse trabecular meshwork using a variety of transgenes associated with glaucoma generates an inducible and reproducible method for generating ocular hypertension due to increased aqueous humor outflow resistance of the conventional outflow pathway. Both adenovirus serotype 5 (Ad5) and lentiviruses have selective tropism for the mouse trabecular meshwork with intraocular injections. Accurate intraocular pressures are easily measured using a rebound tonometer, and aqueous humor outflow facilities can be measured in anesthetized live mice.

Keywords: Glaucoma; Intraocular pressure; Mouse models; Viral transducing vectors.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Aqueous Humor / metabolism
Glaucoma*
Intraocular Pressure
Mice
Ocular Hypertension* / genetics
Ocular Hypertension* / metabolism
Trabecular Meshwork / metabolism

Abstract

Publication types

MeSH terms

Grants and funding