A non-rational approach to optimized targeting of CRISPR-Cas9 complexes

Cell Chem Biol. 2023 Aug 17;30(8):855-857. doi: 10.1016/j.chembiol.2023.07.012.

Abstract

In this issue of Cell Chemical Biology, Bush et al.1 report an in vitro selection method for optimizing CRISPR-Cas9 single-guide RNAs. This approach may be useful in targeting previously intractable genomic sequences. The results also provide insights into which positions in single-guide RNAs are most amenable to modification.

Publication types

  • Comment

MeSH terms

  • CRISPR-Cas Systems* / genetics
  • Genomics*
  • RNA, Guide, CRISPR-Cas Systems

Substances

  • RNA, Guide, CRISPR-Cas Systems