Identification and Validation of an Aging-Associated circRNA-miRNA-mRNA Network in Neovascular Age-Related Macular Degeneration

Gerontology. 2023;69(10):1218-1231. doi: 10.1159/000531287. Epub 2023 Aug 21.

Abstract

Introduction: Neovascular age-related macular degeneration (NVAMD) is a leading cause of severe vision impairment in the elderly. Aging is one of the most pivotal underlying molecular mechanisms of NVAMD.

Methods: In this study, we identified the potential aging-related genes involved in NVAMD. Considering that noncoding RNAs are vital regulators of NVAMD progression, we further explored and constructed an aging-originated circRNA-miRNA-mRNA network of NVAMD. Differential expression of 23 aging-associated genes was identified based on sequencing data and the Human Aging Genomic Resources tool at a threshold of p < 0.05, and log2|fold change| > 1.

Results: We screened 12 microRNAs (miRNAs) using public datasets and miRNet database. A total of 13 circRNAs were subsequently mined using the starBase tool. Merging these 13 circRNAs, 12 miRNAs, and 15 genes together, we obtained 281 pairs of circRNA-miRNA and 30 pairs of miRNA-mRNA.

Conclusion: We created an aging-related circRNA-miRNA-mRNA network, which could be a promising target for future AMD treatments.

Keywords: Age-related macular degeneration; Aging; Circular RNA; MicroRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Gene Expression Profiling
  • Gene Regulatory Networks
  • Humans
  • MicroRNAs* / genetics
  • RNA, Circular / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • MicroRNAs
  • RNA, Circular
  • RNA, Messenger

Grants and funding

This study was supported by the National Natural Science Foundation of China (82171076) and Shanghai Hospital Development Center (SHDC2020CR5014).