Establishment of cancer cell line originating from a patient with high-grade serous ovarian carcinoma

Future Sci OA. 2023 Jul 29;9(8):FSO875. doi: 10.2144/fsoa-2023-0025. eCollection 2023 Sep.

Abstract

Aim: Ovarian cancer is a serious malignancy with high prevalence and mortality.

Methods: We isolated and characterized an ovarian high-grade serous cancer cell line (M4) from a tumor of a Vietnamese patient with ovarian carcinoma.

Results: The M4 cancer cell line showed good proliferation and stability in culture. Morphologically, the M4 cells showed similar characteristics to tumor cells such as a polyhedral shape, large irregular nuclei, high nuclear/cytoplasmic ratio, high nuclear density and expressing cancer markers like CA125, p53 and Ki67 markers.

Conclusion: We have successfully isolated and characterized the M4 cell line from a Vietnamese patient with ovarian carcinoma.

Keywords: CD46; cell line; high-grade serous carcinoma; immunohistochemistry; ovarian cancer.