Four undescribed triterpenes from the aerial parts of Verbena officinalis

Verbena officinalis is used as a Chinese folk medicine for the treatment of rheumatism and bronchitis. Herein, four undescribed triterpenes, officinalisoids A-D (1-4), together with thirty-three known compounds (5-37) were isolated from the aerial parts of V. officinalis. The chemical structures of the new compounds were determined by spectrometric data interpretation using NMR, HRESIMS, IR and UV spectroscopy. Biological evaluation results revealed that compound 30 exhibited potential anti-inflammatory activity with IC₅₀ value of 6.07 μM (CC₅₀ > 50 μM) and compound 12 showed moderate anti-dengue virus activity with the IC₅₀ value of 24.55 μM (CC₅₀ > 50 μM).