Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing

Aaqib Sohail; Carolyn H Baloh; Jonathan Hacker; Laura Cho; Tessa Ryan; Regan W Bergmark; Stella E Lee; Alice Maxfield; Rachel Roditi; Daniel F Dwyer; Kathleen M Buchheit; Tanya M Laidlaw

doi:10.1002/alr.23275

Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing

Int Forum Allergy Rhinol. 2024 May;14(5):972-976. doi: 10.1002/alr.23275. Epub 2023 Sep 23.

Authors

Aaqib Sohail^{1

2}, Carolyn H Baloh^{1

2}, Jonathan Hacker², Laura Cho², Tessa Ryan², Regan W Bergmark³, Stella E Lee³, Alice Maxfield³, Rachel Roditi³, Daniel F Dwyer^{1

2}, Kathleen M Buchheit^{1

2}, Tanya M Laidlaw^{1

2}

Affiliations

¹ Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA.
² Division of Allergy and Clinical Immunology, Brigham and Women's Hospital, Boston, Massachusetts, USA.
³ Division of Otolaryngology-Head & Neck Surgery, Department of Surgery, Harvard Medical School, Brigham and Women's Hospital, Boston, Massachusetts, USA.

PMID: 37742089
DOI: 10.1002/alr.23275

Abstract

Mast cell numbers were reduced in samples cryopreserved as whole tissue chunks. Thawed epithelial cells had reduced proliferation rates when preserved as dissociated cell suspensions. The right cryopreservation method to choose may depend on the goals and cell-type focus of the project.

Keywords: eosinophilic rhinitis and nasal polyposis; innate immunity; paranasal sinus diseases; rhinosinusitis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Proliferation
Cryopreservation* / methods
Epithelial Cells / physiology
Female
Humans
Male
Mast Cells* / physiology
Nasal Polyps*
Sequence Analysis, RNA
Single-Cell Analysis