Gold nanoparticle dimer-based immunochromatography for in situ ultrasensitive detection of porcine epidemic diarrhea virus

Xinrui Shi; Yaxiang Luo; Hao Yan; Guangzhao Tian; Songxin Yang; Zhaoyuan He; Fuxin Zhang; Yueliang Wang; Longhua Guo; Hailan Chen

doi:10.1007/s00604-023-06013-5

Gold nanoparticle dimer-based immunochromatography for in situ ultrasensitive detection of porcine epidemic diarrhea virus

Mikrochim Acta. 2023 Oct 7;190(11):430. doi: 10.1007/s00604-023-06013-5.

Authors

Xinrui Shi^{1

2}, Yaxiang Luo^{1

2}, Hao Yan^{1

3}, Guangzhao Tian^{1

2}, Songxin Yang^{1

3}, Zhaoyuan He¹, Fuxin Zhang^{1

3}, Yueliang Wang⁴, Longhua Guo⁵, Hailan Chen^{6

7

8}

Affiliations

¹ College of Animal Science and Technology, Guangxi University, Nanning, 530005, Guangxi, China.
² Guangxi Zhuang Autonomous Region Engineering Research Center of Veterinary Biologics, Nanning, 530004, Guangxi, China.
³ Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China.
⁴ Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China. [email protected].
⁵ Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China. [email protected].
⁶ College of Animal Science and Technology, Guangxi University, Nanning, 530005, Guangxi, China. [email protected].
⁷ Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, 314001, China. [email protected].
⁸ Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, Nanning, 530004, Guangxi, China. [email protected].

PMID: 37804452
DOI: 10.1007/s00604-023-06013-5

Abstract

The low detection sensitivity of lateral-flow immunochromatography assay (LFIA) based on spherical gold nanoparticle (AuNP) limits its wide applications. In the present study, AuNP dimers with strong plasma scattering and robust signal output were synthesized via the Ag ion soldering (AIS) strategy and used as labeled probes in LFIA to boost the sensitivity without any extra operation process and equipment. The established LFIA exhibited high sensitivity with a limit of detection (LOD) of 2.0 × 10² TCID₅₀/mL for PEDV, which provides 50 times higher sensitivity than commercial LFIA based on spherical colloidal gold. In addition, the AuNP dimer-based LFIA showed strong specificity, good reproducibility, high stability, and good accordance to reverse transcription polymer chain reaction (RT-PCR) when detecting 109 clinical samples. Thus, the AuNP dimers is a promising probe for LFIA and the developed AuNP dimer-based LFIA is suitable for the rapid detection of PEDV in the field.

Keywords: AuNP dimers; In situ detection; Lateral-flow immunochromatography assay; Porcine epidemic diarrhea virus; Spherical nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromatography, Affinity
Gold
Metal Nanoparticles* / chemistry
Polymers
Porcine epidemic diarrhea virus*
Reproducibility of Results
Sensitivity and Specificity
Swine
Swine Diseases* / diagnosis

Substances

Gold
Polymers