Temporal disparity of action potentials triggered in axon initial segments and distal axons in the neocortex

Sci Adv. 2023 Oct 13;9(41):eade4511. doi: 10.1126/sciadv.ade4511. Epub 2023 Oct 12.

Abstract

Neural population activity determines the timing of synaptic inputs, which arrive to dendrites, cell bodies, and axon initial segments (AISs) of cortical neurons. Action potential initiation in the AIS (AIS-APs) is driven by input integration, and the phase preference of AIS-APs during network oscillations is characteristic to cell classes. Distal regions of cortical axons do not receive synaptic inputs, yet experimental induction protocols can trigger retroaxonal action potentials (RA-APs) in axons distal from the soma. We report spontaneously occurring RA-APs in human and rodent cortical interneurons that appear uncorrelated to inputs and population activity. Network-linked triggering of AIS-APs versus input-independent timing of RA-APs of the same interneurons results in disparate temporal contribution of a single cell to in vivo network operation through perisomatic and distal axonal firing.

MeSH terms

  • Action Potentials / physiology
  • Axon Initial Segment*
  • Axons / physiology
  • Dendrites / physiology
  • Humans
  • Neocortex* / physiology