Dalbergiella welwitschia (Baker) Baker f. alkaloid-rich extracts attenuate liver damage in streptozotocin-induced diabetic rats

B O Ajiboye; S Dada; H O Fatoba; O E Lawal; O H Oyeniran; O Y Adetuyi; A Olatunde; M Taher; J Khotib; D Susanti; B E Oyinloye

doi:10.1016/j.biopha.2023.115681

Dalbergiella welwitschia (Baker) Baker f. alkaloid-rich extracts attenuate liver damage in streptozotocin-induced diabetic rats

Biomed Pharmacother. 2023 Dec:168:115681. doi: 10.1016/j.biopha.2023.115681. Epub 2023 Oct 12.

Authors

B O Ajiboye¹, S Dada², H O Fatoba³, O E Lawal³, O H Oyeniran⁴, O Y Adetuyi³, A Olatunde⁵, M Taher⁶, J Khotib⁷, D Susanti⁸, B E Oyinloye⁹

Affiliations

¹ Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria; Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria. Electronic address: [email protected].
² Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Nigeria.
³ Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
⁴ Functional Foods, Nutraceuticals, and Phytomedicine Unit, Department of Biochemistry, Federal University Oye-Ekiti, Ekiti State, Nigeria.
⁵ Phytomedicine and Natural Products Drug Discovery, Department of Medical Biochemistry, Abubakar Tafawa Balewa University, Bauchi, Bauchi State, Nigeria.
⁶ Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia; Pharmaceutics and Translational Research Group, Kulliyyah of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
⁷ Department of Pharmacy Practice, Faculty of Pharmacy, Airlangga University, 60115 Surabaya, Indonesia. Electronic address: [email protected].
⁸ Department of Chemistry, Kulliyyah of Science, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
⁹ Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria; Phytomedicine, Biochemical Toxicology and Biotechnology Research Laboratories, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Nigeria; Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, South Africa.

PMID: 37837880
DOI: 10.1016/j.biopha.2023.115681

Abstract

This experiment was conducted to evaluate the Dalbergiella welwitschia alkaloid-rich extracts on liver damage in streptozotocin-induced diabetic rats. Hence, to induce diabetes, 45 mg/kg body weight of streptozotocin was intraperitoneally injected into the Wistar rats. Subsequently, 5 % (w/v) of glucose water was given to the induced animals for 24 h. Thus, the animals (48) were grouped into five groups (n = 8), containing normal control (NC), diabetic control (DC), diabetic rats placed on low (50 mg/kg body weight) and high (100 mg/kg body weight) doses of D. welwitschi alkaloid-rich leaf extracts (i.e. DWL and DWH respectively), and diabetic rats administered 200 mg/kg body weight of metformin (MET). The animals were sacrificed on the 21st day of the experiment, blood and liver were harvested, and different liver damage biomarkers were evaluated. The results obtained demonstrated that diabetic rats administered DWL, DWH and MET significantly (p < 0.05) increased hepatic AST, ALT, albumin, SOD, CAT, GSH, and GPX levels when compared to DC with no significant (p > 0.05) different when compared with NC. Also, diabetic rats administered DWL, DWH and MET revealed a significant (p < 0.05) decrease in GGT and MDA levels, as well as, fragmented DNA and protein carbonyl levels when compared to DC with no significant (p > 0.05) different when compared with NC. In addition, histological examination revealed that diabetic rats placed on DWL, DWH and MET normalized the hepatocytes. Consequently, it can be inferred that alkaloid-rich extracts from D. welwitschi leaf could be helpful in improving liver damage associated with diabetes mellitus rats.

Keywords: Alkaloid; Dalbergiella welwitschi; Damage; Diabetes mellitus; Liver.

MeSH terms

Alkaloids* / pharmacology
Alkaloids* / therapeutic use
Animals
Blood Glucose / metabolism
Body Weight
Diabetes Mellitus, Experimental* / metabolism
Hypoglycemic Agents / adverse effects
Liver / metabolism
Liver Diseases* / metabolism
Metformin* / pharmacology
Oxidative Stress
Plant Extracts / adverse effects
Rats
Rats, Wistar
Streptozocin / adverse effects

Substances

Streptozocin
Plant Extracts
Alkaloids
Metformin
Blood Glucose
Hypoglycemic Agents