Co-inoculation of phosphate-solubilizing bacteria and phosphate accumulating bacteria in phosphorus-enriched composting regulates phosphorus transformation by facilitating polyphosphate formation

Yabin Zhan; Shaoqi Xu; Zhuonan Hou; Xin Gao; Jing Su; Bihui Peng; Jinyue Zhao; Zhigang Wang; Meidi Cheng; Ake Zhang; Yanbin Guo; Guochun Ding; Ji Li; Yuquan Wei

doi:10.1016/j.biortech.2023.129870

Co-inoculation of phosphate-solubilizing bacteria and phosphate accumulating bacteria in phosphorus-enriched composting regulates phosphorus transformation by facilitating polyphosphate formation

Bioresour Technol. 2023 Dec:390:129870. doi: 10.1016/j.biortech.2023.129870. Epub 2023 Oct 14.

Authors

Yabin Zhan¹, Shaoqi Xu², Zhuonan Hou³, Xin Gao³, Jing Su⁴, Bihui Peng³, Jinyue Zhao³, Zhigang Wang⁵, Meidi Cheng⁶, Ake Zhang⁷, Yanbin Guo³, Guochun Ding³, Ji Li², Yuquan Wei⁸

Affiliations

¹ Key Laboratory of Fertilization from Agricultural Wastes, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064, China; College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China.
² College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China.
³ College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China.
⁴ Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing 210042, China.
⁵ DBN Agriculture Science and Technology Group CO., Ltd., DBN Pig Academy, Beijing 102629, China.
⁶ College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
⁷ College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China; Fuyang Academy of Agricultural Sciences, Fuyang 236065, China.
⁸ College of Resources and Environmental Science, Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, 100193 Beijing, China; Organic Recycling Institute (Suzhou) of China Agricultural University, Wuzhong District, Suzhou 215128, China. Electronic address: [email protected].

PMID: 37839642
DOI: 10.1016/j.biortech.2023.129870

Abstract

This study aimed to explore the impact of co-inoculating phosphate-solubilizing bacteria (PSB) and phosphate accumulating bacteria (PAB) on phosphorus forms transformation, microbial biomass phosphorus (MBP) and polyphosphate (Poly-P) accumulation, bacterial community composition in composting, using high throughput sequencing, PICRUSt 2, network analysis, structural equation model (SEM) and random forest (RF) analysis. The results demonstrated PSB-PAB co-inoculation (T1) reduced Olsen-P content (1.4 g) but had higher levels of MBP (74.2 mg/kg) and Poly-P (419 A.U.) compared to PSB-only (T0). The mantel test revealed a significantly positive correlation between bacterial diversity and both bioavailable P and MBP. Halocella was identified as a key genus related to Poly-P synthesis by network analysis. SEM and RF analysis showed that pH and bacterial community had the most influence on Poly-P synthesis, and PICRUSt 2 analysis revealed inoculation of PAB increased ppk gene abundance in T1. Thus, PSB-PAB co-inoculation provides a new idea for phosphorus management.

Keywords: Composting; Metabolism pathway; Microbial inoculation; Phosphorus cycle regulation; Random forest.

MeSH terms

Bacteria / genetics
Composting*
Phosphates* / chemistry
Phosphorus / analysis
Polyphosphates
Soil / chemistry

Substances

Phosphates
Phosphorus
Soil
Polyphosphates