A macrocyclic fluorescent probe for the detection of citrate

Stephen M Butler; Maria Hountondji; Stuart N Berry; Jian Tan; Laurence Macia; Katrina A Jolliffe

doi:10.1039/d3ob01442h

A macrocyclic fluorescent probe for the detection of citrate

Org Biomol Chem. 2023 Nov 1;21(42):8548-8553. doi: 10.1039/d3ob01442h.

Authors

Stephen M Butler^{1

2}, Maria Hountondji^{1

2}, Stuart N Berry¹, Jian Tan^{3

4}, Laurence Macia^{3

4

5}, Katrina A Jolliffe^{1

2

6}

Affiliations

¹ School of Chemistry, The University of Sydney, NSW, 2006, Australia. [email protected].
² Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, 2006, NSW, Australia.
³ The Charles Perkins Centre, The University of Sydney, NSW 2006, Australia.
⁴ School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, NSW 2006, Australia.
⁵ Sydney Cytometry, The University of Sydney, NSW 2006, Australia.
⁶ The University of Sydney Nano Institute (Sydney Nano), The University of Sydney, NSW 2006, Australia.

PMID: 37846461
DOI: 10.1039/d3ob01442h

Abstract

We present a macrocyclic fluorescent probe for the detection of citrate. This receptor binds citrate through hydrogen-bonding interactions in aqueous solutions, and exhibits a turn-on in fluorescence in response to binding. The presence of common biologically relevant dicarboxylate species does not significantly impact the fluorescence response. We have demonstrated the utility of this probe with the staining of murine splenocytes, and identified different basal levels of citrate present in immune cell subsets via flow cytometry analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Citric Acid* / analysis
Fluorescence
Fluorescent Dyes*
Mice

Substances

Fluorescent Dyes
Citric Acid