Microbiological characterization of Pneumocystis jirovecii pneumonia using quantitative PCR from nasopharyngeal specimens: a retrospective study in a Canadian province from 2019 to 2023

J Clin Microbiol. 2023 Nov 21;61(11):e0091323. doi: 10.1128/jcm.00913-23. Epub 2023 Oct 25.

Abstract

Bronchoalveolar lavage is usually employed for molecular diagnosis of Pneumocystis jirovecii but requires a specialized procedure. By contrast, nasopharyngeal (NP) specimens are easily obtained. In this retrospective study of 35 patients with paired NP and bronchoscopy specimens, NP specimens had a 100% negative percent agreement (95% CI 80.5-100) but only 72.2% positive percent agreement (95% CI 46.5-90.3).

Keywords: PCR; Pneumocystis jirovecii pneumonia; bronchoalveolar lavage specimens; nasopharyngeal specimens.

MeSH terms

  • Bronchoalveolar Lavage Fluid / microbiology
  • Canada
  • Humans
  • Pneumocystis carinii* / genetics
  • Pneumonia, Pneumocystis* / diagnosis
  • Pneumonia, Pneumocystis* / microbiology
  • Polymerase Chain Reaction / methods
  • Retrospective Studies
  • Sensitivity and Specificity