Protocol for the expansion of mouse immune effector cells for in vitro and in vivo studies

Thomas Look; Hanna Meister; Michael Weller; Tobias Weiss

doi:10.1016/j.xpro.2023.102700

Protocol for the expansion of mouse immune effector cells for in vitro and in vivo studies

STAR Protoc. 2023 Dec 15;4(4):102700. doi: 10.1016/j.xpro.2023.102700. Epub 2023 Nov 9.

Authors

Thomas Look¹, Hanna Meister², Michael Weller³, Tobias Weiss⁴

Affiliations

¹ Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 Zurich, Switzerland. Electronic address: [email protected].
² Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 Zurich, Switzerland.
³ Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 Zurich, Switzerland; University Hospital Zurich, 8091 Zurich, Switzerland.
⁴ Department of Neurology, Clinical Neuroscience Center, University of Zurich, 8091 Zurich, Switzerland; University Hospital Zurich, 8091 Zurich, Switzerland. Electronic address: [email protected].

Abstract

Reproducible and efficient expansion of different immune effector cells is required for pre-clinical studies investigating adoptive cell therapies against cancer. Here, we provide a protocol for the rapid expansion of mouse T cells, natural killer (NK) cells, and bone-marrow-derived macrophages (BMDMs). We describe steps for αCD3/αCD8 plate coating, isolating splenocytes, and expanding T cells and NK cells. Further, we detail procedures for bone marrow isolation and BMDM differentiation.

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Flow Cytometry; Immunology.

MeSH terms

Animals
Bone Marrow
Killer Cells, Natural*
Mice
Neoplasms* / therapy
T-Lymphocytes