Objective: To establish a automatic headspace gas chromatography-mass spectrometry (HS/GC-MS) method for the determination of 14 volatile organic compounds in urine. Methods: In September 2022, 10 ml urine sample was taken into a 20 ml headspace bottle, balanced for 30 min at 65 ℃, and then detected by HS/GC-MS and quantified by external standard method. Results: The 14 volatile organic compounds showed good linearity at 0.2-8.0 μg/L and 0.1-4.0 μg/L, with correlation coefficients ranging from 0.9956-0.9999. The recoveries were 79.8%-113.1% with relative standard deviations 0.05%-0.27% when three different concentration levels were added. Detection limit was 0.03-0.05 μg/L. Conclusion: The method is simple and convenient, and the recovery and precision meet the requirements. It can be used for the determination of common volatile organic compounds in urine.
目的： 建立自动顶空气相色谱-质谱联用法测定尿液中14种常见挥发性有机物的分析方法。 方法： 于2022年9月，取10 ml尿样于20 ml顶空瓶，65 ℃条件下平衡30 min，采用顶空技术和气相色谱-质谱应用技术检测，外标法定量。 结果： 14种挥发性有机物在0.2~8.0 μg/L和0.1~4.0 μg/L内线性关系良好，相关系数范围为0.995 6~0.999 9，添加3个不同浓度水平，回收率79.8%~113.1%，相对标准偏差0.05%~0.27%，检出限0.03~0.05 μg/L。 结论： 该检测方法操作简单、方便，回收率与精密度均符合要求，可运用于尿中常见挥发性有机物的检测。.
Keywords: Gas chromatography-mass spectrometry; Organic; Urine.