Brain PET Imaging in Small Animals: Tracer Formulation, Data Acquisition, Image Reconstruction, and Data Analysis

Hussein Bdair; Min Su Kang; Julie Ottoy; Arturo Aliaga; Peter Kunach; Thomas A Singleton; Stephan Blinder; Jean-Paul Soucy; Marco Leyton; Pedro Rosa-Neto; Alexey Kostikov

doi:10.1007/978-1-0716-3499-8_16

Brain PET Imaging in Small Animals: Tracer Formulation, Data Acquisition, Image Reconstruction, and Data Analysis

Methods Mol Biol. 2024:2729:269-284. doi: 10.1007/978-1-0716-3499-8_16.

Authors

Hussein Bdair^{1

2

3}, Min Su Kang^{4

5}, Julie Ottoy⁵, Arturo Aliaga^{4

3}, Peter Kunach^{1

4}, Thomas A Singleton^{1

3}, Stephan Blinder^{3

6}, Jean-Paul Soucy^{1

3

6}, Marco Leyton^{1

2

3

7

8}, Pedro Rosa-Neto^{1

4

3}, Alexey Kostikov^{9

10

11

12}

Affiliations

¹ Department of Neurology and Neurosurgery, McGill University, Montreal, QC, Canada.
² Department of Psychiatry, McGill University, Montreal, QC, Canada.
³ McConnell Brain Imaging Centre, Montreal Neurological Institute-Hospital (The Neuro), McGill University, Montreal, QC, Canada.
⁴ Translational Neuroimaging Laboratory, Research Centre for Studies in Aging, Douglas Research Institute, McGill University, Montreal, QC, Canada.
⁵ LC Campbell Cognitive Neurology Unit, Hurvitz Brain Sciences Program, Sunnybrook Research Institute, University of Toronto, Toronto, ON, Canada.
⁶ PERFORM Centre, Concordia University, Montreal, QC, Canada.
⁷ Department of Psychology, McGill University, Montreal, QC, Canada.
⁸ Center for Studies in Behavioral Neurobiology, Concordia University, Montreal, QC, Canada.
⁹ Department of Neurology and Neurosurgery, McGill University, Montreal, QC, Canada. [email protected].
¹⁰ Translational Neuroimaging Laboratory, Research Centre for Studies in Aging, Douglas Research Institute, McGill University, Montreal, QC, Canada. [email protected].
¹¹ McConnell Brain Imaging Centre, Montreal Neurological Institute-Hospital (The Neuro), McGill University, Montreal, QC, Canada. [email protected].
¹² Department of Chemistry, McGill University, Montreal, QC, Canada. [email protected].

PMID: 38006502
DOI: 10.1007/978-1-0716-3499-8_16

Abstract

Positron emission tomography (PET) is a noninvasive functional imaging modality that involves in vivo detection of spatiotemporal changes in the binding of radioactive pharmaceuticals (a.k.a. PET tracers) to their target sites in different organs. The development of new PET tracers commonly involves their preclinical evaluation in small rodents. Moreover, laboratory animal PET research is now being used with progressively greater frequency to complement human PET studies, to investigate in greater depth the underlying pathophysiology of human diseases, and to monitor the efficiency of novel therapeutic interventions. Here we describe the steps toward a successful small animal PET study, from tracer formulation and image acquisition to data reconstruction and analysis of the acquired scans, with a particular focus on its utility for the brain.

Keywords: PET image acquisition; PET image analysis; PET image reconstruction; PET tracers; Positron emission tomography; Preclinical imaging.

MeSH terms

Animals
Brain* / diagnostic imaging
Data Analysis
Humans
Image Processing, Computer-Assisted / methods
Positron-Emission Tomography* / methods
Radiopharmaceuticals

Substances

Radiopharmaceuticals