Comparative Performance of Anyplex II HPV28 and Cobas 4800 Human Papillomavirus (HPV) Assays for High-Risk HPV Detection in Self-collected Anal Samples

Gauthier Delvallez; Sokleaph Cheng; Stéphane Marot; Gervillien Arnold Malonga; Théophile Cocherie; Steve Wignall; Vincent Calvez; Sophat Phal; Kem Vichet; Anne-Geneviève Marcelin; Aude Jary

doi:10.1093/ofid/ofad540

Comparative Performance of Anyplex II HPV28 and Cobas 4800 Human Papillomavirus (HPV) Assays for High-Risk HPV Detection in Self-collected Anal Samples

Open Forum Infect Dis. 2023 Oct 31;10(11):ofad540. doi: 10.1093/ofid/ofad540. eCollection 2023 Nov.

Affiliations

¹ Medical Biology Laboratory, Institut Pasteur du Cambodge, Phnom Penh, Cambodia.
² Sorbonne Université, Inserm, Institut Pierre Louis d'Epidémiologie et de Santé Publique, Assistance Publique-Hôpitaux de Paris, Service de Virologie, Hôpital Pitié-Salpêtrière, Department of Virology, Paris, France.
³ Family Health International, Phnom Penh, Cambodia.
⁴ Men's Health Cambodia, Phnom Penh, Cambodia.

Abstract

We compared 2 human papillomavirus (HPV) assays to detect the 14 high-risk HPV (hrHPV) genotypes in self-collected anal samples. We found a good agreement and similar performance to detect HPV-16, HPV-18, and the 12 other hrHPV genotypes. The global performance to detect the 14 hrHPV genotypes was not significantly different between the 2 assays.

Keywords: Anyplex II HPV-28; Cobas 4800; HPV testing; anal lesions; self-sampling.