Metabolomic and lipidomic studies on the intervention of taurochenodeoxycholic acid in mice with hyperlipidemia

Na Cui; Wensen Zhang; Fazhi Su; Zhihong Zhang; Biao Li; Donghui Peng; Yanping Sun; Yuanning Zeng; Bingyou Yang; Haixue Kuang; Qiuhong Wang

doi:10.3389/fphar.2023.1255931

Metabolomic and lipidomic studies on the intervention of taurochenodeoxycholic acid in mice with hyperlipidemia

Front Pharmacol. 2023 Nov 15:14:1255931. doi: 10.3389/fphar.2023.1255931. eCollection 2023.

Authors

Affiliations

¹ Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin, China.
² School of Chinese Materia Medica, Guangdong Engineering Technology Research Center for Standardized Processing of Chinese Materia Medica Guangdong Pharmaceutical University, Guangdong, China.

Abstract

Bile acids are the main component of animal bile and are directly involved in the metabolic process of lipids in vivo. Taurochenodeoxycholic acid (TCDCA) is the primary biologically active substance in bile acids and has biological functions such as antioxidant, antipyretic, anti-inflammatory, and analgesic activities and improves immunity. In the present study, we assessed the impact of TCDCA on hyperlipidemia development in mouse models. Mice were fed a high-fat diet (HFD) to induce hyperlipidemia and orally administered different doses of TCDCA orally for 30 days. Then, indicators such as triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in mice were detected. Using HE and ORO staining techniques, the morphology of the mice's liver tissue was detected. Based on metabolomic and lipidomic analyses, we determined the mechanism of TCDCA in treating hyperlipidemia. The results showed that TCDCA had a significant ameliorating effect on dietary hyperlipidemia. In addition, it exerted therapeutic effects through glycerophospholipid metabolism.

Keywords: glycerophospholipid metabolism; hyperlipidemia; lipidomics; metabolomics; taurochenodeoxycholic acid.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. This study was supported by the Chief Scientist of Qi-Huang Project of National Traditional Chinese Medicine Inheritance and Innovation “One Hundred Million” Talent Project (2021); the Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program (2018); Heilongjiang Touyan Innovation Team Program (2019); the National Famous Old Traditional Chinese Medicine Experts Inheritance Studio Construction Program of National Administration of TCM (Grant Number: [2022] No. 75); and the Seventh Batch of National Famous Old Traditional Chinese Medicine Experts Experience Heritage Construction Program of National Administration of TCM (Grant Number: [2022] No. 76). This work was financially supported by the Administration of Traditional Chinese Medicine of Guangdong Province, China (20221214).